Simultaneous Estimation of Aspirin and Vonoprazan in Pure and Pharmaceutical Dosage Form by UV Spectroscopy

Abstract

A simple, accurate, and precise UV-visible spectrophotometric method was developed for the simultaneous estimation of Aspirin and Vonoprazan in pure and pharmaceutical formulations. The method utilized the simultaneous equation method based on their respective absorbance maxima at 225 nm Aspirin and 247 nm Vonoprazan. Validation as per ICH Q2(R1) guidelines showed linearity over 10–50 μg/ml for Aspirin and 2–10 μg/ml for Vonoprazan, with correlation coefficients of 0.9968 and 0.9991, respectively. The method demonstrated excellent specificity, precision (%RSD < 2%), accuracy (mean recovery ~100%), and sensitivity, with low LOD and LOQ values. The developed method was successfully applied to marketed formulations with high accuracy.